Rapid clear® cap 3000

產品介紹:

The Thomson Optimum Growth™ Family of products is expanding into downstream processing with a revolutionary new technology that allows high speed clarification of cellular material. The filters work by simply switching the vented Optimum Growth™ Cap to the Rapid Clear® Cap. The only equipment required is a peristaltic pump. In initial testing the units can filter up to 2.5L-3L of high density culture, 6×10^6 -20×10^6 @ >70% viability, in approximately 18 minutes. This technique will transform the time consuming and laborious task of harvesting cells to a rapid walk away procedure.

規格訊息:

Rapid clear® cap 3000 學士帽造型過濾蓋

High-speed clarification of 2–4 L of cell culture for use with 2.8 & 5 L Optimum Growth™ Flasks

 

1. 無需預先離心-在35分鐘內就可過濾3 L的細胞培養液 比起傳統方式快上13倍

 

2. 一顆抵四顆傳統過濾杯

 

3. 無需倒出換瓶-濾蓋直接接在 Optimum Growth™ Flasks上

 

4. 0.22 μm filter

 

 

     
操作步驟:
 
1. 將細胞培養在2.8 L或5 L的Optimum Growth™ Flask
2. 將flask移至無菌操作台中並將透氣蓋替換成Rapid clear® cap
3. 接收端的蓋子接在1.6、2.8或5 L的Optimum Growth Flask上 (視液體體積量與實驗需求而定)
4. 將兩瓶flask移出無菌操作台,並將中央的矽膠管接在蠕動幫浦上,打開幫浦並設定高速
5. 可輕搖clear cap端的flask並略傾斜,使培養液盡可能的被幫浦帶到接收瓶中
6. 視需要可再加入400 mL的PBS至Optimum Growth™ Flask做潤洗並再過濾,降低樣品殘留
(降低幫浦轉速=>夾閉矽膠管=>關閉幫浦電源=>打開clear cap蓋加入PBS。另一端可維持負壓)

 

 

參考的使用體積與所需時間         註: 不同細胞總類與濃度,所需時間會略有不同

 Cell Line Viability 99 %-70 % 69 %-50 % 49 %-40 % 39 %-0 %
Spin for 7 min @ 4000 g*
 Cell Type Volume (L) Time (min) Volume (L) Time (min) Volume (L) Time (min) Volume (L) Time (min)
 CHO Stable without Feed 3.0 18 2.5 18 2.0 20 3.5** 35**
 CHO Stable, 1 to 2 Feeds 2.0 18 2.0 18 1.5 35    
 CHO Stable, 2+ Feeds Spin for 15 min @ 3000 g; ≦3 L volume **
 HEK293 (FreeStyleTM & Expi293) 3.0 18 3.0 23 3.0 25 3.5** 35**
 CHO Transient 3.0 18 2.5 18 1.5 35    
 ExpiCHO 3.0 18 2.5 18 1.0 18    

* For low viability cultures, (< 39%), centrifuge for 7 minutes prior to clarifying with the Rapid Clear® Cap 3000.
** Cell cultures that received 2+ feeds will require spinning to minimize potential clogging

 

Rapid Clear® Cap 3000 Specifications
Flask Size  2.8 L & 5 L
Part# 788116
Description High-speed clarification of 2 L–4 L of cell culture | Sterile
Tubing Connection Transfer Cap
Tubing Diameter Size 15 silicone tubing, ID: 3/16” (4.76 mm), OD: 7/16” (11.1 mm)
Tubing  Chemically resistant, flexible
Tubing Length 48” (1219.2 mm)
Cap Material  PP (polypropylene)
Qty/Case  8

 

產品影片:

詢價車:

  • Part NO.
  • Qty
  • Inquiry
  • Rapid clear® cap 3000
  • 788116
  • 8/case
top